Investigation of the inflammatory and oxidative stress-inducing effects of deoxynivalenol and T-2 toxin exposure in non-tumorigenic human intestinal cell model

Fungi in the Fusarium genus produce trichothecene mycotoxins including deoxynivalenol (DON) and T-2 toxin which may elicit their damaging effects on gastrointestinal tract following consumption of contaminated cereal-based foods. The aim our study was to evaluate these commonly occurring fusarotoxins alone combination using human, non-cancerous intestinal epithelial cell line HIEC-6. Based experimental data, 24 h after treatment with fusarotoxins, hydrogen peroxide levels, intracellular oxidative stress amounts inflammatory interleukins IL-6 IL-8 significantly increased. Cell membrane localization tight junction protein claudin-1 decreased, whereas distribution occludin remained unchanged. Taken together, HIEC-6 appears be a suitable model for monitoring combined at cellular level changes redox states cells.